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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become observed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was identified to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two further splice variants were described but in depth nucleotide sequence details is not really still obtainable.
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Blocking peptides are peptides that bind specially into the target antibody and block antibody binding. These peptide normally contains the epitope acknowledged because of the antibody.
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This guideline outlines even more achievable optimization of mobile and immunoassay parameters to be certain the very best effects are...
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This information outlines further more doable optimization of mobile and immunoassay parameters to guarantee the very best benefits are...
Antibodies bound to the blocking peptide no longer bind towards the epitope within the goal protein. By comparing the staining from the blocked antibody vs . the antibody alone, you can see which staining is certain.
Focus on Details Cyclin E belongs to your hugely conserved cyclin household, whose associates are characterized by a remarkable periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic event. Cyclin E varieties a fancy with and capabilities for a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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